TRIP INITIATIVE
  • Home
    • About Us
    • Donate
    • FAQs - Summer TRIP
    • Contact
  • Blog
  • ALUMNI GALLERY
    • Ursinus 2015
    • Temple 2015
    • WTHS 2016
    • Temple 2016
    • WTHS 2017
    • Temple 2017
    • WTHS 2018
    • Temple 2018
    • WTHS 2019
    • Temple 2019
    • WTHS 2020
    • WTHS 2021
    • Temple 2021
    • WTHS 2022
    • Temple 2022
    • WTHS 2023
    • WTHS 2024
    • WTHS 2025
  • WTHS'25 Symposium
    • WTHS'25 Symposium Schedule >
      • Session A Schedule
      • Session B Schedule
  • Donate

Regular updates from students in TRIP

The Finish Line by Diego Asencio

10/4/2021

0 Comments

 
After a bittersweet last day in the lab, my TRIP experience is near over. All the train rides, homework, calculations, and flies are behind me. However, all the knowledge that I have gained and relationships that I have made will never be lost. After the first week, where keeping on top of the homework was not my strong suit, I never thought that I would be able to finish anything in the intensive lab, but with the help of Dr. Leystra and Dr. Purdy, my experiment was a success! Let's take a look into exactly what was a success, what wasn’t, and how I learned from the amazing experience in the TRIP Lab.
Picture
My experiment was set up to answer the question, does omeprazole have any long term side effects? I take omeprazole to help treat my Eosinophilic Esophagitis, so I wanted to make sure that there wouldn't be any major unknown side effects. The Larval Locomotion with Methyl Blue assay was the perfect experiment to use to answer my question. How it works is on a 10 cm x 15 cm strip of thermal paper, I spread out five larvae and painted them with a 0.2% methyl blue dilution. The larvae would leave a trail on the paper showing exactly where they went over a 10 minute period. I then used a software to measure out the distance and used the average distance traveled per condition to determine whether or not the larvae were healthy. I used a control condition, and then by using 2 solutions, I made the equivalent to 20 mg, 40 mg, 80 mg, and 120 mg of omeprazole for flies and put it into their food. The question was successful overall, and depending on how you look at it not so good news - The higher the dose the larvae were given, the less average distance they traveled.
Now, it wouldn’t be a teenager in a university lab without a few mistakes here and there. At first, I wanted to test the lifespan of the fly, but there would not be enough time to conduct the assay in the lab. This was the ultimate goal, as I wanted to make sure that my lifespan would not be affected by this drug, but I was unfortunately never able to test the lifespan. Furthermore, after picking the Larval Locomotion with methyl blue, I wanted to not only measure the distance, but also the amount of turns and how fast the larvae were moving. But the turns seemed to be completely random, and the speed was impossible to measure unless I recorded all 10 minutes of the larvae and tried to estimate the speed. On top of these mistakes, on my second rep I forgot to make a control vial, so now I am completely aware about the dangers of sharing control conditions between different reps, and then I forgot to take flies home with me to conduct the assay at home for rep 5. So all in all, being naive was an incredibly obvious thing to not do, but I fell for the trap of biting off more than I could chew.
Picture
And so now, at the end of it all, what have I learned? To be honest, I will have to see what I learned when I get thrown into another lab experience. Testing my skills such as pipetting, calculations, communications, and clarity, are all going to be important, and that's not to mention getting to the lab on time, having all my work done for the day, finding time to eat and drink, which are all just as important. But there is no doubt in my mind that TRIP has better prepared me for any science lab that I will see in the future. If I could say one thing to my week one self, I would tell him to put his head down and get to work, because TRIP is not a cakewalk.
0 Comments

Your comment will be posted after it is approved.


Leave a Reply.

    Archives

    April 2025
    March 2025
    February 2025
    September 2024
    August 2024
    July 2024
    May 2024
    April 2024
    March 2024
    July 2023
    June 2023
    May 2023
    April 2023
    March 2023
    October 2022
    September 2022
    August 2022
    July 2022
    June 2022
    May 2022
    April 2022
    March 2022
    February 2022
    October 2021
    September 2021
    August 2021
    July 2021
    June 2021
    May 2021
    April 2021
    March 2021
    December 2020
    November 2020
    October 2020
    July 2020
    June 2020
    May 2020
    April 2020
    December 2019
    November 2019
    October 2019
    September 2019
    August 2019
    July 2019
    June 2019
    May 2019
    April 2019
    March 2019
    February 2019
    December 2018
    November 2018
    October 2018
    September 2018
    August 2018
    July 2018
    April 2018
    March 2018
    February 2018
    August 2017
    July 2017

    RSS Feed

    Categories

    All
    Blog 1: Introductions!
    Blog 2: Planning An Independent Project
    Blog 3: Reflections

Thank you to our donors for making our mission possible through your generous support.
Picture

Company

About
Fox Chase Cancer Center

Support

Contact
​Menu
WEBSITE DESIGN INFORMATION:
The TRIP Initiative website was built by Alesandra (Sasha) Temerte (TRIP@TU'17 alum) ​ and Charity Robbins (TRIP@TU'22) and is not a product of Fox Chase Cancer Center
© COPYRIGHT 2023. ALL RIGHTS RESERVED.
WEBSITE DESIGN BY ALESANDRA SASHA TEMERTE & CHARITY ROBBINS
LOGO DESIGN BY ARNAV DHINGRA
Photo from NIH-NCATS
  • Home
    • About Us
    • Donate
    • FAQs - Summer TRIP
    • Contact
  • Blog
  • ALUMNI GALLERY
    • Ursinus 2015
    • Temple 2015
    • WTHS 2016
    • Temple 2016
    • WTHS 2017
    • Temple 2017
    • WTHS 2018
    • Temple 2018
    • WTHS 2019
    • Temple 2019
    • WTHS 2020
    • WTHS 2021
    • Temple 2021
    • WTHS 2022
    • Temple 2022
    • WTHS 2023
    • WTHS 2024
    • WTHS 2025
  • WTHS'25 Symposium
    • WTHS'25 Symposium Schedule >
      • Session A Schedule
      • Session B Schedule
  • Donate