Hi friends! It is hard to believe that TRIP is over. Ten weeks seemed like a lot when we first started, but now we are at the close of the program with the final presentation happening next Saturday. I have learned so much in the lab and thoroughly enjoyed the experiments with fruit flies as I knew exactly what I wanted to do each day. Thanks to Dr. Purdy, Dr. Leystra, and the TAs, I was able to perform my tasks efficiently with the help of the weekly experimental plans.
I set up two control groups and three experimental conditions (atorvastatin only, fenugreek only, both atorvastatin and fenugreek), and flies were fed for 7 days. On the seventh day, glucose level in flies’ blood was measured using Glucose Metabolism Assay. The flies were crushed, and their hemolymph was extracted. As part of the Glucose Metabolism Assay, the hemolymph went through a series of enzymatic reactions resulting in a pink color solution. Then, the color absorbance was measured using a spectrophotometer. This was interesting, but it was a lengthy process that took more than four hours to complete. As expected, atorvastatin increased the blood sugar in flies by 10%. The results were encouraging that fenugreek counteracted atorvastatin’s detrimental effects on glucose level by reducing the blood sugar by 26%.
I have gained a greater appreciation of the scientific research involved in developing medicines and new treatments. I am tremendously grateful for this opportunity for research, and I am looking forward to applying the skills I learned in my future endeavors. Thank you, TRIP, for allowing me to dive deeper into the world of research! This may be the end of an exciting TRIP, but it is the beginning of the next trip.
This may be the end of an exciting TRIP, but it is the beginning of the next trip.