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Regular updates from students in TRIP

Some Gruesome Techniques

8/3/2017

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My name is Sarah Nugent, and I attend Central Bucks High School East. I am a rising senior, and I aspire to pursue a profession in medicine. My favorite subject is biology, and I love studying human anatomy. If I decide to study medicine, then I’d like to explore neurology, pathology, neuroscience, endocrinology, or orthopedics. However, research is also interesting. I love TRIP because it gives students the opportunity to explore the field of research and all that it entails.
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So far with my project, I’ve been studying the impact of intermittent starvation and nutritional supplements on the metabolic activity of fruit flies. Here’s how I do it: first I have to decapitate the given group of flies. After that, I place the bodies in a centrifuge tube and add PBS using something called a micropipette. I love practicing using the micropipette, as it’s a great tool for measuring out small quantities of solution. Next, I crush the bodies in order to extract the hemolymph. Doesn’t this sound gruesome!! After that, I centrifuge the bodies to form a pellet. Using the micropipette, I isolate the liquid from the pellet and add it to a conical tube and add the assay reagent. After incubating the tube at 37oC for 30 minutes, I add sulfuric acid, and measure the absorption of glucose using a spectrophotometer. The process as a whole is very interesting, as I’m learning new research techniques.
 
The program is very educational and allows students to exercise different skills beyond the classroom. If anyone is considering applying, I would highly recommend it.

Author: Sarah Nugent
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  • Home
    • About Us
    • FAQ
    • Contact
  • Blog
  • ALUMNI GALLERY
    • Ursinus 2015
    • Temple 2015
    • WTHS 2016
    • Temple 2016
    • WTHS 2017
    • Temple 2017
    • WTHS 2018
  • Apply to TRIP