If flies are exposed to both Celsius and melatonin, will the effects of the Celsius be reversed by the effects of melatonin and have an improvement in cognitive function and memory?
After I developed questions and formulated a topic I was interested in, I realized I wanted to use the larval memory assay, which measures the cognitive functions of a fruitful larva. I began to dive into the work of experimental design and calculating drug stocks. Using the mass of an average male human and the wet mass of a fruit fly with the recommended amount of Celsius and melatonin, I was able to calculate how much of each substance was to be added into my fly food. The math was definitely a challenge and I was pretty confused with all the small numbers and steps, but with the help of Dr. Leystra and Dr. Purdy, as well as many of the new friends I made here at TRIP, I was able to calculate the necessary amount and easily made the drug stocks using micropipettes and other materials. Though I spend about 4 hours every Saturday, I’ve realized over the past couple of weeks here at TRIP, that time goes by so fast! The people I am with are very supportive and give great advice. They will help you sort flies, grab materials, and double check your drug calculations. I’m so excited to see how my experiment turns out and if the results are similar to my predictions! I look forward to spending more time with my friends and my TRIP family. Thank you for taking the time to read about my experiences here at TRIP and I hope to keep you guys updated on my findings!
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