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Regular updates from students in TRIP

The Trials and Tribulations of Science

8/1/2017

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Hi everyone! My name is Kayla Kinsler, and I am one of the 15 participants in the TRIP program. I am a night owl, and when school is in session, I am up all night studying which leaves me exhausted for the next day. I am not a fan of coffee so this gave me the idea to test the effect of energy drinks (which would be my next option) on the flies. 

On the first week of independent projects, I tested the effect energy drinks had on metabolism using 15 males from each of the 4 vials I had (control, monster energy drink, 5 hour energy drink, and red bull). I came up with the idea to sleep deprive my remaining flies by sticking them in front of the light, and I planned to test their memory to simulate me staying up all night and then taking a test the next day after drinking an energy drink. However, this was a huge mistake because all the flies ended up dying in the light, and I realized that I had no control in this experiment that I could've compared results with, which is a huge problem

When I came into the lab the next day to find my flies dead, I felt extremely defeated and like I had failed myself and Dr. Purdy. The TRIP staff was nothing but supportive and Dr, Purdy even gave me the idea to try testing starved flies reaction to short term exposure to the drink in petri dishes. That has been my experience so far at TRIP. It has been nothing but continuous support from each of the wonderful staff members, and I feel as though everyone is rooting for my success. I have also had the opportunity to learn various lessons on lab techniques and science in general. It is truly a family environment, and I am excited to come into the lab each day, and I am sad to see it end, but I am also excited for those who are to come after me.

Author: Kayla Kinsler

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Laughs & Smiles

8/1/2017

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Hello!
 
My name is Sofiya Lysenko, and I am rising sophomore attending Abington Senior High School in Abington, PA. This summer, I’m super excited to be a part of TRIP! I’ve learned so much in the past few weeks, from how to contain the flies’ excitement to explore the word outside of their hackneyed vials to how to measure the amount of glucose in their hemolymph.
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For my project, I’m using an Arduino (a microcontroller), breadboard, and jumper wires to try and simulate an electrically polluted environment for the flies. I’m treating some vials simultaneously with varying degrees of turmeric, an antioxidant, to see if it can reverse the effects. I’m really excited to be investigating these conditions because we are exposed to them every day and the health effects are still not entirely clear. Plus, I get to do two of my favorite things at once - study Drosophila and wire circuits!! :)
Although science is super exciting and fun most of the time, it doesn’t always go as planned in the process. The instructors have all been really supportive, kind, and patient during all these weeks. Not only are they always willing to lend a hand in times of peril, but they also are helping cultivate our passions for science and grow as both scientists and students. Their enthusiasm is contagious and class has never gone by without lots of laughs and smiles in between!!
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Author: Sofiya Lysenko
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What Did I Get Myself Into?!

8/1/2017

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When I realized that I will be an interviewee for the TRIP Initiative Program, I had to brainstorm how to make myself seem great! I started to revise things that I would say and how to act. For example, giving a stern handshake and saying, “Hi, I am Sheril Pinnock. Nice to meet you! ...etc.” The usual stuff. Then it hit me, ‘What if you get picked, what will be your experiment? Hmm. BPA! Eureka!'
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My name is Sheril Pinnock and my experiment is, ‘The Effect of Bisphenol A on Drosophila Melanogaster (AKA- the fruit fly).’ I am 8 days into being a TRIP student and I have learned so much about the job of a research scientist and some of the equipment they use. Also, I have experienced what it feels like to be harassed by the paparazzi because thanks to Ms. Smith and whoever takes pictures, I have also discovered another reason for why I hate taking pictures.
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Back to my experiment: Why I chose to use BPA is to find out how being exposed to BPA can affect the human body (specifically fertility in females and metabolism is males), because everyone is ingesting or touching something that has BPA but doesn’t know that it could be extremely harmful. With the continued help of Dr. Purdy and all the wonderful TA’s, I hope to find something that should scare me and my audience. I could have never had a better summer!!
 
P.S. I really don’t like that last picture, but I think it goes well to show off the childish science geek in me.

Author: Sheril Pinnock
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Fruit Fly Fun

8/1/2017

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Hello! My name is Saket Gokhale, and I’m a rising junior at Methacton High School. Some of my favorite things to do are play tennis, watch sports, and watch action films. I have a fervent interest in the sciences and hope to pursue a career in medicine!
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My TRIP experience is one that cannot be replicated. I never thought that I would be working with fruit flies, especially since I try to kill flies when they come in the house. However, I understand the importance fruit flies serve to the scientific community due to their surprising similarities to humans. We spent our first few sessions exploring techniques such as sorting, transferring, and making fly food and grape plates. For my first screen, I investigated the effect of fenugreek and head trauma on the activity of the fly. I inflicted head trauma using the apparatus and then conducted the negative geotaxis assay, which I thought was an innovative way to gauge activity. I found that the drug and the head trauma balance each other out in terms of fly mobility.
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For my independent project, I wanted to look into the effects of supplements that could help alleviate the symptoms of diabetes since my family has a history of this disorder. I am researching the effects of natural ingredients on the glucose levels of the fruit fly. First, I’m exposing the flies to regular and high sugar food. Then I am splitting these two groups into five: regular food, sugary food, food with fenugreek, food with cinnamon, and food with turmeric. This way, I can also assess any short term and long term changes with exposure to the different types of food. I’m using the glucose assay as a measurement procedure. I’m looking forward to know the results! Throughout the process, I have received tremendous help and guidance. Thanks to the amazing mentors and helpers who made this journey a valuable learning experience to cherish! 

Author: Saket Gokhale

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